The Resource Affinity probe capillary electrophoresis evaluation of aptamer binding to Campylobacter jejuni bacteria, by Dimitra N. Stratis-Cullum, Sun McMasters, and Paul M. Pellegrino, (electronic resource)

Affinity probe capillary electrophoresis evaluation of aptamer binding to Campylobacter jejuni bacteria, by Dimitra N. Stratis-Cullum, Sun McMasters, and Paul M. Pellegrino, (electronic resource)

Label
Affinity probe capillary electrophoresis evaluation of aptamer binding to Campylobacter jejuni bacteria
Title
Affinity probe capillary electrophoresis evaluation of aptamer binding to Campylobacter jejuni bacteria
Statement of responsibility
by Dimitra N. Stratis-Cullum, Sun McMasters, and Paul M. Pellegrino
Creator
Contributor
Subject
Language
eng
Summary
In this work, we developed a capillary electrophoresis immunoassay to evaluate aptamer binding to bacterial cells. This work is the first known demonstration of aptamer affinity probe capillary electrophoresis to a whole cell target. The aptamer probes investigated were developed against a Campylobacter jejuni bacterial cell target, and we evaluated binding relative to other common food-borne pathogens. Specific binding affinity was evidenced by a pronounced mobility shift and peak broadening with increasing bacteria concentration. Little to no mobility shift was observed for food-borne pathogens, Salmonella typhirium and Escherichia coli, even when increasing concentrations 10-fold over target. These results suggest that affinity probe capillary electrophoresis could be useful for qualitative screening of aptamer candidates to bacterial cell targets
Member of
Cataloging source
DTICE
http://library.link/vocab/creatorName
Stratis-Cullum, Dimitra N
Government publication
federal national government publication
Illustrations
illustrations
Index
no index present
Literary form
non fiction
Nature of contents
  • dictionaries
  • bibliography
Period covered
2007-2008.
http://library.link/vocab/relatedWorkOrContributorName
  • McMasters, Sun
  • Pellegrino, Paul M
  • U.S. Army Research Laboratory
Series statement
ARL-TR
Series volume
5015
http://library.link/vocab/subjectName
  • Medicine and medical research
  • Microbiology
  • Pathogenic microorganisms
  • Jejunum
  • Escherichia coli
  • Immunoassay
  • Food
  • Bioassay
  • Electrophoresis
  • Salmonella
  • Shifting
  • Capillarity
  • Bacteria
  • Campylobacter jejuni
  • Electrophoresis
  • Immunoassay
Target audience
adult
Type of report
Progress report;
Label
Affinity probe capillary electrophoresis evaluation of aptamer binding to Campylobacter jejuni bacteria, by Dimitra N. Stratis-Cullum, Sun McMasters, and Paul M. Pellegrino, (electronic resource)
Link
Instantiates
Publication
Note
  • Title from title screen (viewed on Nov. 11, 2012)
  • "November 2009."
Bibliography note
Includes bibliographical references (p. 9-12)
Color
mixed
Control code
713205230
Dimensions
unknown
Extent
1 online resource (vi, 14 p.)
Form of item
online
Governing access note
  • APPROVED FOR PUBLIC RELEASE
  • APPROVED FOR PUBLIC RELEASE
Other physical details
ill. (some col.).
Specific material designation
remote
Label
Affinity probe capillary electrophoresis evaluation of aptamer binding to Campylobacter jejuni bacteria, by Dimitra N. Stratis-Cullum, Sun McMasters, and Paul M. Pellegrino, (electronic resource)
Link
Publication
Note
  • Title from title screen (viewed on Nov. 11, 2012)
  • "November 2009."
Bibliography note
Includes bibliographical references (p. 9-12)
Color
mixed
Control code
713205230
Dimensions
unknown
Extent
1 online resource (vi, 14 p.)
Form of item
online
Governing access note
  • APPROVED FOR PUBLIC RELEASE
  • APPROVED FOR PUBLIC RELEASE
Other physical details
ill. (some col.).
Specific material designation
remote

Library Locations

  • Central LibraryBorrow it
    200 SE Martin Luther King Jr. Blvd., Evansville, IN, 47713, US
    37.971461 -87.565988
  • East BranchBorrow it
    840 E Chandler Ave., Evansville, IN, 47713, US
    37.965908 -87.550480
  • McCollough BranchBorrow it
    5115 Washington Ave., Evansville, IN, 47715, US
    37.961977 -87.488508
  • North Park BranchBorrow it
    960 Koehler Dr., Evansville, IN, 47710, US
    38.026947 -87.577202
  • Oaklyn BranchBorrow it
    3001 Oaklyn Dr., Evansville, IN, 47711, US
    38.010718 -87.511553
  • Red Bank BranchBorrow it
    120 S Red Bank Rd., Evansville, IN, 47712, US
    37.977988 -87.636409
  • Stringtown BranchBorrow it
    2100 Stringtown Rd., Evansville, IN, 47711, US
    37.996832 -87.555245
  • West BranchBorrow it
    2000 W Franklin St., Evansville, IN, 47712, US
    37.979875 -87.594773
Processing Feedback ...